Active Recombinant Human Fms-related Tyrosine Kinase 3, Fc Chimera

• Cat.No.: FLT3-140H
• Product Overview: Recombinant Human fms-related tyrosine kinase 3 encoding the signal peptide and extracellular domain of human Flt-3 (aa 1-540) was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-540 a.a.
• Description: Fms-like tyrosine kinase 3 (Flt-3) and its cognate ligand, Flt-3 Ligand (Flt-3L), are essential growth factors involved in the survival and differentiation of hematopoietic progenitor and stem cells, specifically the development of NK cells, pre-B and pre-T cells, monocytes/macrophages and dendritic cells.
• Amino Acid Sequence: NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGTDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDRIPKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK.
• Molecular Mass: Flt-3-Fc Chimera migrates as a broad band between 100 and 130 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation.
• pI: Flt-3-Fc Chimera separates into a number of isoforms with a pI between 5.1 and 6.8 in 2D PAGE due to post-translational modifications, in particular glycosylation.
• % Carbohydrate: Purified Flt-3-Fc Chimera consists of 15-35% carbohydrate by weight.
• Glycosylation: Flt-3-Fc Chimera has N-linked and O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: The ED50 of Flt-3-Fc Chimera is typically 1.0 -2.0 ng/ml as measured by its ability to neutralize Flt-3 mediated proliferation of the OCI/AML5 human leukemia cell line.

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Synonyms: FLT3; fms-related tyrosine kinase 3; FLK2; STK1; CD135; CD135 antigen; fetal liver kinase 2; FL cytokine receptor; tal liver kinase 2; stem cell tyrosine kinase 1; FLT3 receptor tyrosine kinase; tyrosine-protein kinase receptor FLT3; growth factor receptor tyrosine kinase type III; C 2.7.10.1; OTTHUMP00000042340; fetal liver kinase 2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• UniProt ID: P36888
• Chromosome Location: 13q12
• MIM: 136351
• Pathway: ute myeloid leukemia; Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Pathways in cancer
• Function: ATP binding; nucleotide binding; osphoinositide 3-kinase binding; protein binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity