Active Recombinant Human Folate Receptor 3 (Gamma), His-tagged

• Cat.No.: FOLR3-557H
• Product Overview: Recombinant Human FOLR3protein was expressed in Murine myeloma cell line with a C-terminal 6-His tag.Ala22-Ser245 & Ser26-Ser245.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 22-245;26-245 a.a.
• Description: Folate receptor 3(FOLR3), also known as folate receptor γ, is a 32 kDa protein that bindsfolic acid and reduced folates. Dietary folates are required for many keymetabolic processes including nucleotide and methionine synthesis, theinterconversion of glycine and serine, and histidine breakdown. Mature FOLR3is a glycosylated protein that contains an imperfect carboxy terminal GPIanchor sequence. A fraction of FOLR3 is GPIlinked to the cell surface, butthe majority is released as a secreted protein. Mature human FOLR3 shares 74%and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Ala22 & Ser26
• Molecular Weight: 26 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When Folic Acid-Bovine SerumAlbumin is coated at 5 μg/mL (100 μL/ well), the concentration of rhFOLR3that produces 50% of the optimal binding response is found to beapproximately 0.2-1 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 30-40kDa, reducing conditions
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: FOLR3 folate receptor 3 (gamma)[ Homo sapiens ]
• Official Symbol: FOLR3
• Synonyms: FOLR3;folate receptor 3 (gamma); FR-G; FR-gamma; gamma-hFR; folate receptor gamma;Folate receptor 3; OTTHUMP00000237135
• Gene ID: 2352
• mRNA Refseq: NM_000804
• Protein Refseq: NP_000795
• MIM: 602469
• UniProt ID: P41439
• Chromosome Location: 11q13
• Function: folic acid binding;receptor activity