Species : |
Human |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Gln23-Ser245 |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Folic acid-BSA immobilized on CM5 Chip can bind Human FOLR3, His Tag with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200). |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 27.4 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |