Active Recombinant Human G Protein-Coupled Receptor Associated Sorting Protein 1, His-tagged
Cat.No. : | GPRASP1-5633H |
Product Overview : | RecombinantHuman GPRASP1 protein was expressed in Murine myeloma cellline. Leu35-His576,with a C-terminal 6-His tag. |
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Cat. No. : | GPRASP1-5633H |
Description : | GPRASP1 was isolated in a screen to identify proteins in micethat copurify with myostatin (GDF-8), a potent negative regulator of skeletalmuscle mass. The GPRASP1 cDNA encodes a 571amino acid protein that contains a 29 amino acid signal sequence and iscomprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin,two tandem Kunitz, and netrin. Two related human proteins which contain thesame domain structure are called WFIKKN (based on the presence and order ofthese conserved domains) and WFIKKNRP (WFIKKN-related protein). |
Source : | Mammalian cells |
Form : | Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein. |
N-terminalSequence : | Leu35 |
MolecularWeight : | 60.7 kDa |
Activity : | Measuredby its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenousleukemia cells. The ED50 for this effect is typically 0.03-0.15 μg/mL, in thepresence of 40 ng/mL of rmGDF-8. Measured by its ability to inhibit trypsin cleavageof a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 valueis approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nMtrypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5. |
Endotoxin Level : | <1.0EU per 1μg of the protein by the LAL method. |
SDS-PAGE : | 75-80kDa, reducing conditions. |
Purity : | >95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : | 10 μg/mLin sterile PBS containing at least 0.1% human or bovine serum albumin |
Storage : | Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C understerile conditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |
Protein length : | Leu35-His576 |
Gene Name : | GPRASP1 G protein-coupledreceptor associated sorting protein 1 [ Homo sapiens ] |
Official Symbol : | GPRASP1 |
Synonyms : | GPRASP1; Gprotein-coupled receptor associated sorting protein 1; GASP;GASP1; GASP-1; G-protein coupled receptor-associated sorting protein 1; KIAA0443 |
Gene ID : | 9737 |
mRNA Refseq : | NM_001184727 |
Protein Refseq : | NP_001171656 |
MIM : | 300417 |
UniProt ID : | Q5JY77 |
Chromosome Location : | Xq22.1 |
Function : | protein binding |
Products Types
◆ Recombinant Protein | ||
GPRASP1-3896M | Recombinant Mouse GPRASP1 Protein, His (Fc)-Avi-tagged | +Inquiry |
GPRASP1-3070H | Recombinant Human GPRASP1 Protein, His (Fc)-Avi-tagged | +Inquiry |
GPRASP1-2326R | Recombinant Rat GPRASP1 Protein, His (Fc)-Avi-tagged | +Inquiry |
GPRASP1-2672R | Recombinant Rat GPRASP1 Protein | +Inquiry |
GPRASP1-7214M | Recombinant Mouse GPRASP1 Protein | +Inquiry |
◆ Lysates | ||
GPRASP1-5772HCL | Recombinant Human GPRASP1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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