Active Recombinant Human GDNF protein(Arg83-Ile185)
Cat.No. : | GDNF-7292H |
Product Overview : | Recombinant Human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified in HEK293, with additional two aa (Gly& Pro) at the N-terminus. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | N/A |
Protein length : | Arg83-Ile185 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured in a cell prolifeRation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1 / GDNFRA Protein (His Tag), the EC50 is 15-48 ng/mL. |
Molecular Mass : | The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migRates as approximately 18 KDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 97 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | GDNF glial cell derived neurotrophic factor [ Homo sapiens ] |
Official Symbol : | GDNF |
Synonyms : | GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; astrocyte derived trophic factor; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF; |
Gene ID : | 2668 |
mRNA Refseq : | NM_000514 |
Protein Refseq : | NP_000505 |
MIM : | 600837 |
UniProt ID : | P39905 |
Products Types
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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