Active Recombinant Human Glutaredoxin 1

• Cat.No.: grxA-48H
• Product Overview: Purified from E.coli expressing the human Glutaredoxin 1 gene (1-106 a.a.)

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-106 a.a.
• Description: Glutaredoxin 1 catalyzes GSH-disulfide oxidoreductase.
• Form: Frozen liquid. In storage buffer composed of 20 mM HEPES, pH 7.0, 10% glycerol and 1 mM DTT.
• Bio-activity: 130 U/mg
• Molecular Mass: 11.8 kDa
• Purity: >95% as determined by SDS-PAGE
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: Store at -80°C. Avoid repeated freeze/thaw cycles.
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Citation

Catalytic Cleavage of Disulfide Bonds in Small Molecules and Linkers of Antibody-Drug Conjugates.

Journal: Drug metabolism and disposition: the biological fate of chemicals    PubMed ID: 31085544    Data: 2020/6/1

Authors: Donglu Zhang, Aimee Fourie-O'Donohue, S Cyrus Khojasteh

Article Snippet:109 110 Materials and Methods: 111 Materials 112 Ammonium formate, formic acid, NADPH, human recombinant human TRX, rat liver TRX 113 reductase, and a proprietary TRX reductase inhibitor (catalog # T9199) were purchased 114 from Sigma-Aldrich (St. Louis, MO, USA).109 110 Materials and Methods: 111 Materials 112 Ammonium formate, formic acid, NADPH, human recombinant human TRX, rat liver TRX 113 reductase, and a proprietary TRX reductase inhibitor (catalog # T9199) were purchased 114 from Sigma-Aldrich (St. Louis, MO, USA).. Human GRX I, rat recombinant TRX, and GRX 115 reductase were purchased from Creative BioMart (Shirley, NY).. Compounds 1-10 and 12 116 at A SPE T Journals on M ay 16, 2019 dm d.aspetjournals.org D ow nloaded from were made as described previously (Pei et al., 2018).Compounds 1-10 and 12 116 at A SPE T Journals on M ay 16, 2019 dm d.aspetjournals.org D ow nloaded from were made as described previously (Pei et al., 2018).