Active Recombinant Human GPC5 protein, His-tagged

• Cat.No.: GPC5-3332H
• Product Overview: Active Recombinant Human GPC5 protein(NP_004457.1) (Met 1-Thr 554) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-554 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5 μg/ml (100 μl/well) can bind human bFGF with a linear ranger of 0.156-2.5 ng/ml.
• Molecular Mass: The secreted recombinant human GPC5 consists of 540 amino acids and predicts a molecular mass of 60.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GPC5 glypican 5 [ Homo sapiens ]
• Official Symbol: GPC5
• Synonyms: GPC5; glypican 5; glypican-5; glypican proteoglycan 5; bA93M14.1
• Gene ID: 2262
• mRNA Refseq: NM_004466
• Protein Refseq: NP_004457
• MIM: 602446
• UniProt ID: P78333