Active Recombinant Human GPNMB Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: GPNMB-747HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human GPNMB (Accession # NP_002501) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 2.5 - 10 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human GPNMB, Fc Chimera has a calculated MW of 78.6 kDa (monomer). In SDS-PAGE migrates as 115-125 kDa, reducing conditions.
• N-terminal Sequence Analysis: Lys 23
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: GPNMB glycoprotein (transmembrane) nmb [ Homo sapiens ]
• Official Symbol: GPNMB
• Synonyms: GPNMB; glycoprotein (transmembrane) nmb; transmembrane glycoprotein NMB; glycoprotein NMB; glycoprotein nmb like protein; HGFIN; NMB; osteoactivin; transmembrane glycoprotein; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN
• Gene ID: 10457
• mRNA Refseq: NM_001005340
• Protein Refseq: NP_001005340
• MIM: 604368
• UniProt ID: Q14956