Active Recombinant Human GPNMB Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: GPNMB-747HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human GPNMB (Accession # NP_002501) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 2.5 - 10 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human GPNMB, Fc Chimera has a calculated MW of 78.6 kDa (monomer). In SDS-PAGE migrates as 115-125 kDa, reducing conditions.
• N-terminal Sequence Analysis: Lys 23
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.

Gene Information

• Gene Name: GPNMB glycoprotein (transmembrane) nmb [ Homo sapiens ]
• Official Symbol: GPNMB
• Synonyms: GPNMB; glycoprotein (transmembrane) nmb; transmembrane glycoprotein NMB; glycoprotein NMB; glycoprotein nmb like protein; HGFIN; NMB; osteoactivin; transmembrane glycoprotein; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN
• Gene ID: 10457
• mRNA Refseq: NM_001005340
• Protein Refseq: NP_001005340
• MIM: 604368
• UniProt ID: Q14956

Reviews

06/29/2022

The potency and power of this protein met my expectations.

02/01/2022

There are no adverse reactions and few side effects.

08/08/2021

The quality of this one is very good, and they also have a long lifespan, and I am very satisfied with this product.

Q&As

How to prepare GPNMB recombinant protein in the experiment?

In the experiment, the GPNMB gene can be introduced into the expression vector by gene cloning technology, and then the GPNMB recombinant protein can be expressed in Escherichia coli or eukaryotic cells.

How to use animal models to study the function and disease mechanism of GPNMB recombinant protein?

Animal models carrying human mutant genes can be constructed using transgenic or gene editing technology to study the role and disease mechanism of GPNMB recombinant protein in vivo. These animal models can be used to study the effects of GPNMB recombinant proteins on tumor growth, metastasis, and drug resistance.

What are the treatments for GPNMB recombinant protein?

The current treatments for GPNMB recombinant protein mainly include gene therapy, protein replacement therapy, and drug therapy for cell surface receptors and signal transduction processes.

What is the significance of GPNMB recombinant protein in tumor diagnosis and treatment?

By detecting the expression level and modification status of GPNMB recombinant protein, it can be used to diagnose certain tumor diseases. In addition, the recombinant protein of GPNMB may also become a new target for the treatment of tumors, providing new ideas and methods for tumor treatment.

How does GPNMB recombinant protein interact with other proteins?

GPNMB recombinant protein can interact with other proteins, such as cell surface receptors such as integrin, and participate in cell signal transduction processes.

If the level of GPNMB recombinant protein is abnormal, what should be treated?

If the level of GPNMB recombinant protein is abnormal, the treatment may include the use of drugs or other special treatments, depending on the condition and the doctor's recommendation.