Active Recombinant Human GPR77 Full Length Transmembrane protein(Nanodisc)

• Cat.No.: GPR77-19478H
• Product Overview: Recombinant Human GPR77 Full Length Transmembrane protein(Q9P296), was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Form: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Bio-activity: Elisa plates were pre-coated with Flag Tag GPR77-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR77-Nanodisc is 3.415ng/ml.
• Molecular Mass: The human full length C5AR2 protein has a MW of 36.1 kDa.
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Full Length: Full L.

Gene Information

• Gene Name: GPR77 G protein-coupled receptor 77 [ Homo sapiens ]
• Official Symbol: GPR77
• Synonyms: GPR77; G protein-coupled receptor 77; C5a anaphylatoxin chemotactic receptor C5L2; C5L2; G-protein coupled receptor 77; G protein-coupled receptor C5L2; GPF77
• Gene ID: 27202
• mRNA Refseq: NM_018485
• Protein Refseq: NP_060955
• MIM: 609949
• UniProt ID: Q9P296