Active Recombinant Human GTF2E1/GTF2E2, His-tagged

• Cat.No.: GTF2E1-8477H
• Product Overview: Recombinant Human GTF2E1/GTF2E2,fused with His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The human Transcription Factor IIE (TFIIE) is composed of 56 kDa (a) and 34 kDa (?) subunits and is shown to be a heterotetramer . The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain . TFIIE binds to RNA polymerase II in solution and joins the pre-initiation complex probably concomitant with RNA polymerase II and TFIIF .
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 20 units are sufficient for an in vitro reconstituted transcription assay; 100 units are sufficient for a protein-protein interaction assay.
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 500 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: GTF2E1 general transcription factor IIE, polypeptide 1, alpha 56kDa [ Homo sapiens ]
• Official Symbol: GTF2E1
• Synonyms: general transcription factor IIE, polypeptide 1, alpha 56kDa; GTF2E1; FE; TF2E1; TFIIE-A; general transcription factor IIE subunit 1; TFIIE-alpha; general transcription factor IIE 56 kDa subunit; transcription initiation factor IIE subunit alpha
• Gene ID: 2960
• mRNA Refseq: NM_005513
• Protein Refseq: NP_005504
• MIM: 189962
• UniProt ID: P29083
• Chromosome Location: 3q21-q24
• Pathway: Basal transcription factors, organism-specific biosystem; Epstein-Barr virus infection, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: metal ion binding; protein binding; sequence-specific DNA binding; zinc ion binding; sequence-specific DNA binding; zinc ion binding