Active Recombinant Human HAVCR1 Protein, His-Tagged

• Cat.No.: HAVCR1-01H
• Product Overview: Mouse myeloma cell line, NS0-derived human TIM-1/KIM-1/HAVCR protein
  Ser21-Thr288, with a C-terminal 6-His tag

Specification

• Description: The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. The protein is a receptor for multiple other viruses, including Ebola virus, Marburg virus, Dengue virus, and Zika virus and is a possible entry factor for SARS-CoV-2 and other coronaviruses.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL
  anti-CD3 and Recombinant Human TIM‑1/KIM‑1/HAVCR.
  The ED50 for this effect is 0.2-0.8 µg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: 29.6 kDa
• N-terminal Sequence Analysis: Ser21
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 °C as supplied.
  1 month, 2 to 8 °C under sterile conditions after reconstitution.
  3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: HAVCR1 hepatitis A virus cellular receptor 1 [ Homo sapiens (human) ]
• Official Symbol: HAVCR
• Synonyms: CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1
• Gene ID: 26762
• mRNA Refseq: NM_001173393.3
• Protein Refseq: NP_001166864.1
• MIM: 606518
• UniProt ID: Q96D42