Active Recombinant Human HEXB protein, His-tagged

• Cat.No.: HEXB-302H
• Product Overview: Recombinant Human HEXB protein(P07686)(Ala43-Met556), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ala43-Met556
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze 4methylumbelliferylNacetylβDglucosaminide(4MUGlcNAc). The specific activity is > 3000 pmols/min/μg.
• Molecular Mass: The recombinant human HEXB comprises 525 amino acids and has a predicted molecular mass of 60.2 kDa. The apparent molecular mass of the protein is approximately 60-66 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HEXB hexosaminidase B (beta polypeptide) [ Homo sapiens ]
• Official Symbol: HEXB
• Synonyms: HEXB; hexosaminidase B (beta polypeptide); beta-hexosaminidase subunit beta; HCC-7; hexosaminidase subunit B; beta-N-acetylhexosaminidase subunit beta; cervical cancer proto-oncogene 7 protein; N-acetyl-beta-glucosaminidase subunit beta; ENC-1AS
• Gene ID: 3074
• mRNA Refseq: NM_000521
• Protein Refseq: NP_000512
• MIM: 606873
• UniProt ID: P07686