Active Recombinant Human HGF Protein (Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile), GMP Grade

• Cat.No.: HGF-05HG
• Product Overview: GMP recombinant human HGF protein (Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile) without tag was expressed in Mouse myeloma cell line and manufactured and tested under cGMP guidelines.

Specification

• Description: This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
• Source: Mouse myeloma cell line
• Species: Human
• Form: Disulfide-linked heterodimer
• Bio-activity: Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 0.1-0.5 ng/mL.
  The specific activity of recombinant human HGF is >1.0 x 106 IU/mg, which is calibrated against the human HGF WHO International Standard.
• Molecular Mass: Predicted Molecular Mass: alpha chain: 53.7 kDa beta chain: 26 kDa
  SDS-PAGE: 65-75 kDa, non-reducing conditions; 60-65 kDa, and 30-40 kDa, reducing conditions
• Protein Length: Gln32-Ser728, Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile
• N-terminal Sequence Analysis: Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32
  Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu ( alpha -chain)
  Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn ( beta -chain)
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
• Official Symbol: HGF
• Synonyms: HGF; hepatocyte growth factor (hepapoietin A; scatter factor); deafness, autosomal recessive 39 , DFNB39; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; lung fibroblast derived mitogen; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; F-TCF; DFNB39
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210