Active Recombinant Human HPGD protein, His-tagged

• Cat.No.: HPGD-881H
• Product Overview: Active Recombinant Human HPGD protein(NP_000851.2) (Met 1-Gln 266) was expressed in E. coli, with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: E. coli
• Tag: His
• Protein Length: 1-266 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM DTT, 10% glycerol, pH 7.5.
• Bio-activity: Measure by the production of NADH during the oxidation of PGF2α. The specific activity is >1,500 pmoles/min/μg.
• Molecular Mass: The recombinant human HPGD comprises 272 amino acids and has a predicted molecular mass of 29.7 kDa. It migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000851.2
• Unit ID: P15428
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P15428

Gene Information

• Gene Name: HPGD hydroxyprostaglandin dehydrogenase 15-(NAD) [ Homo sapiens ]
• Official Symbol: HPGD
• Synonyms: HPGD; hydroxyprostaglandin dehydrogenase 15-(NAD); 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; SDR36C1; short chain dehydrogenase/reductase family 36C; member 1; prostaglandin dehydrogenase 1; NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 36C, member 1; PGDH; PGDH1; PHOAR1; 15-PGDH
• Gene ID: 3248
• mRNA Refseq: NM_000860
• protein Refseq: NP_000851
• MIM: 601688