Active Recombinant Human HPSE protein, His-tagged

• Cat.No.: HPSE-431H
• Product Overview: Recombinant Human HPSE(Gln36-Ile543), fused with His tag at N-terminal was expressed in CHO. The linker peptide from Ser110-Gln157 is removed by protease treatment.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 36-543;110-157 a.a.
• Predicted N Terminal: His & K158
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and E64.
• Bio-activity: Measured by its ability to release heparan sulfate from Recombinant Human Syndecan-4. <60 ng of Recombinant Human Active Heparanase/HPSE is required for the release of 50% of heparan sulfate from 2 μg of Recombinant Human Syndecan-4, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 43 & 9 kDa;SDS-PAGE: 50-60 kDa & 7-9 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: HPSE heparanase [ Homo sapiens ]
• Official Symbol: HPSE
• Synonyms: HPSE; heparanase; HPA; HPSE1; HSE1; heparanase-1; endo-glucoronidase; HPA1; HPR1
• Gene ID: 10855
• mRNA Refseq: NM_006665
• Protein Refseq: NP_006656
• MIM: 604724
• UniProt ID: Q9Y251
• Chromosome Location: 4q21.3
• Pathway: Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Heparan sulfate degradation, organism-specific biosystem; Heparan sulfate degradation, conserved biosystem; Metabolic pathways, organism-specific biosystem; Syndecan-1-mediated signaling events, organism-specific biosystem
• Function: beta-glucuronidase activity; cation binding; hydrolase activity, acting on glycosyl bonds; protein binding; protein dimerization activity; syndecan binding