Active Recombinant Human HTRA1, His-tagged

• Cat.No.: HTRA1-2891H
• Product Overview: Recombinant human HTRA1(Gly156­Pro480) fused with 6*his tag at C-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 156-480 a.a.
• Description: HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family. The E. coli HTRA homolog functions as a chaperoneproteaseand is essential for bacterial survival at temperatures above 42 °C. Among the four mammalian HTRA proteins, HTRA1, 3and 4are secreted while HTRA2 islocalized in mitochondria. HTRA1 contains an Nterminalinsulin likegrowth factor binding protein (IGFBP) domain, a Kazaltypetrypsin inhibitor motif, and C terminaltrypsin likeprotease and PDZ domains. It is involved in several pathologies including Alzheimer's disease, rheumatoid arthritis, osteoarthritis andagerelatedmacular degeneration, although the mechanisms by which HTRA1 exerts its effects are not clear. HTRA1 also has properties of a tumor suppressorprotein, which makes it a target for cancer therapy. In addition HTRA1 is known to regulate the TGFβsignaling pathway and bone mineralization.
• NterminalSequenceAnalysis: Met
• MolecularMass: 36kDa
• Activity: Measured by its ability to cleave βcasein.>95% of βCaseinis cleaved by Recombinant Human HTRA1/PRSS11.
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, as determined by SDS-PAGE.
• Formulation: Supplied as a 0.2 μm filtered solution in MES and NaCl.
• Stability& Storage: Use a manual defrost freezer and avoid repeated freeze-thawcycles.6 months from date of receipt, 20to 70°C as supplied.3 months, 20to 70°C under sterile conditions after opening.

Gene Information

• Gene Name: HTRA1 HtrA serine peptidase 1 [ Homo sapiens ]
• Official Symbol: HTRA1
• Synonyms: HTRA1; HtrA serine peptidase 1; L56; HtrA; ARMD7; ORF480; PRSS11; serine protease HTRA1; IGFBP5-protease; protease, serine, 11 (IGF binding); HTRA; Serine protease 11; EC 3.4.21.-
• Gene ID: 5654
• mRNA Refseq: NM_002775
• Protein Refseq: NP_002766
• MIM: 602194
• UniProt ID: Q92743
• Chromosome Location: 10q26.3
• Function: insulin-like growth factor binding; peptidase activity; serine-type endopeptidase activity