Active Recombinant Human HTRA2 protein

• Cat.No.: HTRA2-2589H
• Product Overview: Recombinant Human HTRA2 protein(Ala134-Glu458) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 134-458 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Bio-activity: Protease activity demonstrated by HtrA2 cleavage of bovine β-casein. Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.
• Molecular Mass: 34.98 kDa
• Endotoxin: Please contact us for more information.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: HTRA2 HtrA serine peptidase 2 [ Homo sapiens ]
• Official Symbol: HTRA2
• Synonyms: HTRA2; HtrA serine peptidase 2; protease, serine, 25 , PRSS25; serine protease HTRA2, mitochondrial; OMI; PARK13; serine protease 25; protease, serine, 25; serine proteinase OMI; HtrA-like serine protease; Omi stress-regulated endoprotease; high temperature requirement protein A2; PRSS25
• Gene ID: 27429
• mRNA Refseq: NM_013247
• Protein Refseq: NP_037379
• MIM: 606441
• UniProt ID: O43464