Active Recombinant Human ICAM1 protein, His-tagged

• Cat.No.: ICAM1-2249H
• Product Overview: Recombinant Human ICAM1 (Met 1-Glu 480) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 μg/ml, 100 μl/well), approximately 30%-60% will adhere specifically.
• Molecular Mass: The recombinant human ICAM1 consists of 464 amino acids and has a predicted molecular mass of 51 kDa. As a result of glycosylation, the apparent molecular mass of rhICAM1 is approxiamtely 65-70 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met 1-Glu 480
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ICAM1 intercellular adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: ICAM1
• Synonyms: ICAM1; intercellular adhesion molecule 1; BB2; CD54; human rhinovirus receptor; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; P3.58
• Gene ID: 3383
• mRNA Refseq: NM_000201
• Protein Refseq: NP_000192
• MIM: 147840
• UniProt ID: P05362

Reviews

04/27/2022

Manufacturers may provide documentation of the protein's validation, which includes information about its purity, concentration, stability, and potential contaminants.

11/05/2020

This can be particularly important in clinical trials to ensure accurate and reliable results.

10/14/2016

If the need arises for larger quantities of ICAM1 protein during the later stages of clinical trials, the manufacturer can facilitate scale-up production processes to meet those requirements.

Q&As

Can ICAM1 be a potential therapeutic target?

Yes, targeting ICAM1 is being explored as a potential therapeutic strategy for inflammatory diseases, with research focusing on developing ICAM1 inhibitors.

How is ICAM1 involved in inflammation?

ICAM1 is upregulated during inflammation, promoting the adhesion of immune cells to the endothelium and facilitating their migration into inflamed tissues.

How is ICAM1 involved in cancer progression?

ICAM1 expression can be altered in cancer cells and is implicated in promoting cancer progression by facilitating tumor cell adhesion, invasion, and metastasis.

Are there diagnostic applications for ICAM1 in clinical settings?

ICAM1 levels can be measured in blood or tissue samples, and elevated levels may serve as diagnostic markers for certain inflammatory diseases.

Can ICAM1 be used as a prognostic marker in certain diseases?

Elevated levels of ICAM1 in some diseases may serve as prognostic markers, indicating disease severity and potential outcomes.