Species : |
Human |
Source : |
HEK293 |
Tag : |
Fc |
Protein Length : |
24-189 a.a. |
Form : |
Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Loaded Human IFN-alpha / beta R1, His Tag on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human IFNAR2, His Tag on HIS1K Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 2.67 nM as determined in BLI assay (ForteBio Octet Red96e). |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 45.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Cys24-Glu189 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |