| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
24-166 aa |
| Form : |
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1. 97 ng/mL. 2. Measured in a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 0.74-2.96 ng/mL, corresponding to a specific activity of 3.38×10^5~1. 35×10^6 units/mg. 3. Flow cytometry: 1X106A549 cells treated with IFN-γ (1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (5 μl/Test,right).4.Flow cytometry: 1X106NCI-H460 cells treated with IFN-γ (1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (5 μl/Test,right) |
| Molecular Mass : |
16.78 kDa |
| Endotoxin : |
< 0.1 EU/μg of the protein by LAL method. |
| Purity : |
≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC. |
| Storage : |
Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
| Reconstitution : |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
