Active Recombinant Human IFNG Protein (Gln24-Gln166), GMP Grade, Animal-Free

• Cat.No.: IFNG-30HG
• Product Overview: GMP recombinant human IFN-gamma protein (Gln24-Gln166) with an N-terminal Met was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Gln24-Gln166
• Description: This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
• Bio-activity: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.15-0.75 ng/mL.
  The specific activity of recombinant human IFN-gamma is >2.0 x 107 IU/mg, which is calibrated against the human IFN-gamma Standard.
• Molecular Mass: Predicted Molecular Mass: 16.9 kDa
  SDS-PAGE: 17 kDa, reducing conditions
• N-terminal Sequence Analysis: Met-Gln24-Asp-Pro-Tyr-Val-Lys-Glu-Ala-Glu
• Endotoxin: <0.01 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
• Reconstitution: Reconstitute at 200 μg/mL in sterile, deionized water.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: IFNG interferon, gamma [ Homo sapiens ]
• Official Symbol: IFNG
• Synonyms: IFNG; interferon, gamma; interferon gamma; IFN-gamma; immune interferon; IFG; IFI
• Gene ID: 3458
• mRNA Refseq: NM_000619
• Protein Refseq: NP_000610
• MIM: 147570
• UniProt ID: P01579