Active Recombinant Human IGF2 insulin-like growth factor 2 (somatomedin A)

• Cat.No.: IGF2-371H
• Product Overview: Recombinant Human IGF-II produced in E.coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7.505 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilmstumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5'' region overlaps the INS gene and the 3'' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Tyr-Arg-Pro-Ser.
• Purity: Purity > 97% by (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver-Stained gel.
• Activity: rHuIGF-II is fully biologically active when compared to standards.The ED50, calculated by the competitive binding of IGF-II plasma derived to human placental membrane is < 1.0 ng/ml, corresponding to a specific activity of 106 U/mg.
• Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm.2. Analysis by RP-HPLC, using a calibrated solution of IGF-II as a Reference Standard.
• Storage: -20oC, avoid repeated freeze and thaw cycles.
• Pathways: Diabetes pathways
• Publications:
  Control of Adipose Tissue Expandability in Response to High Fat Diet by the Insulin-like Growth Factor-binding Protein-4 (2014)

Gene Information

• Gene Name: IGF2 insulin-like growth factor 2 (somatomedin A) [ Homo sapiens ]
• Official Symbol: IGF2
• Synonyms: insulin-like growth factor 2 (somatomedin A);INSIGF; pp9974; C11orf43; FLJ22066; FLJ44734; IGF2; insulin-like growth factor 2; IGF-II; somatomedin A; somatomedin-A; insulin-like growth factor II; insulin-like growth factor type 2; putative insulin-like growth factor II associated protein; chromosome 11 open reading frame 43; OTTHUMP00000011157; OTTHUMP00000011018
• Gene ID: 3481
• mRNA Refseq: NM_000612
• Protein Refseq: NP_000603
• MIM: 147470
• UniProt ID: P01344
• Chromosome Location: 11p15.5
• Function: growth factor activity; hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; protein binding

Citation

Control of Adipose Tissue Expandability in Response to High Fat Diet by the Insulin-like Growth Factor-binding Protein-4

Journal: The Journal of Biological Chemistry    PubMed ID: 24778188    Data: 2014/6/27

Authors: Olga Gealekman, Kunal Gurav, Silvia Corvera

Article Snippet:In experiments comparing insulin, IGF-1 and IGF-2 action, three-dimensional images of each well were captured using an automated microscopy system as described ( 24 ), and the area of growth surrounding each explant was quantified using automated image analysis protocols.In experiments comparing insulin, IGF-1 and IGF-2 action, three-dimensional images of each well were captured using an automated microscopy system as described ( 24 ), and the area of growth surrounding each explant was quantified using automated image analysis protocols.. IGF-1 and IGF-2 were obtained from Creative BioMart and used at concentrations indicated in A . fig ft0 fig mode=article f1 FIGURE 8. caption a4 Effect of IGF-1 on sprout formation.. A , effects of insulin, IGF-1 and IGF-2, and of IGFbp4 on the percent of explants displaying capillary sprouts after 14 days in culture.A , effects of insulin, IGF-1 and IGF-2, and of IGFbp4 on the percent of explants displaying capillary sprouts after 14 days in culture.