Active Recombinant Human IGFBP2, Met-tagged

• Cat.No.: IGFBP2-477H
• Product Overview: Recombinant Human IGFBP2 (Accession # CAA34373) Glu40-Gln328, fused with an N-terminal Met, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Protein Length: 40-328 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.03 - 0.09 µg/mL in the presence of 14 ng/mL rhIGF-II.
• Molecular Mass: Recombinant Human IGFBP2, Met-tagged has a calculated MW of 31 kDa. In SDS-PAGE migrates as 36 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IGFBP2 insulin-like growth factor binding protein 2, 36kDa [ Homo sapiens ]
• Official Symbol: IGFBP2
• Synonyms: IGFBP2; insulin-like growth factor binding protein 2, 36kDa; IBP2, insulin like growth factor binding protein 2 (36kD); insulin-like growth factor-binding protein 2; IBP-2; IGFBP-2; IGF-binding protein 2; IBP2; IGF-BP53
• Gene ID: 3485
• mRNA Refseq: NM_000597
• Protein Refseq: NP_000588
• MIM: 146731
• UniProt ID: P18065
• Chromosome Location: 2q33-q34