Active Recombinant Human IgG2Fc protein

• Cat.No.: IgG2Fc-0214H
• Product Overview: Recombinant Human IgG2Fc protein(Glu99-Lys326) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 99-326 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Human FCGRT&B2M Heterodimer Protein, His Tag captured on CM5 Chip via anti-His antibody can bind Human IgG2 Fc, Tag Free with an affinity constant of 41.9 nM as determined in SPR assay (Biacore 8K).
• Molecular Mass: This protein carries no "tag".The protein has a calculated MW of 25.7 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu99-Lys326
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: IgG2Fc