Active Recombinant Human IgG4Fc protein

• Cat.No.: IgG4Fc-1574H
• Product Overview: Recombinant Human IgG4Fc protein(Glu99-Lys327) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 99-327 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5.
• Bio-activity: Human FCGRT&B2M Heterodimer Protein, His Tag captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K).
• Molecular Mass: This protein carries no "tag".The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).
• Proteinlength: Glu99-Lys327
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.