Active Recombinant Human IL12B&IL12A protein, His-Avi&Flag-tagged, Biotinylated
Cat.No. : | IL12B&IL12A-0222H |
Product Overview : | Biotinylated Recombinant Human IL12B&IL12A protein(Ile23-Ser328(IL-12B)&Arg23-Ser219(IL-12A)), fused with His and Avi and Flag tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His-Avi&Flag |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized Human IL-12 R beta 1, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag with a linear range of 0.039-0.625 μg/mL.Immobilized Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate. can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL.Immobilized Human IL-12 R beta 2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag with a linear range of 0.003-0.3 μg/mL. |
Molecular Mass : | Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 38.3 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag) and subunit IL-12A is fused with flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 40-50 kDa (IL12B) and 32-35 kDa (IL12A) respectively due to glyosylation. |
Proteinlength : | Ile23-Ser328(IL-12B)&Arg23-Ser219(IL-12A) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ] |
Official Symbol : | IL12B |
Synonyms : | IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B; |
Gene ID : | 3593 |
mRNA Refseq : | NM_002187 |
Protein Refseq : | NP_002178 |
MIM : | 161561 |
UniProt ID : | P29460 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
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