Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human IL12RB1 protein, Fc-Avi-tagged, Biotinylated

Cat.No. : IL12RB1-051H
Product Overview : Biotinylated Recombinant Human IL12RB1(Cys24-Glu540) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells .
  • Specification
  • Gene Information
  • Related Products
Description : IL12RB1 (Interleukin-12 receptor subunit beta-1; IL‑12 R beta 1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).
Source : CHO cells
Species : Human
Tag : Fc/Avi
Predicted N Terminal : Cys24
Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Human IL12 is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL12RB1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.
Molecular Mass : 95-115 kDa, under reducing conditions
Protein length : Cys24-Glu540
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Applications : Bioactivity
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 200 μg/mL in PBS.
Gene Name : IL12RB1 interleukin 12 receptor, beta 1 [ Homo sapiens ]
Official Symbol : IL12RB1
Synonyms : IL12RB1; interleukin 12 receptor, beta 1; IL12RB; interleukin-12 receptor subunit beta-1; CD212; IL-12RB1; IL-12R-beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; interleukin-12 receptor beta-1 chain; IL-12R-BETA1; MGC34454;
Gene ID : 3594
mRNA Refseq : NM_005535
Protein Refseq : NP_005526
MIM : 601604
UniProt ID : P42701

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
Could you explain the significance of CRISPR/Cas9-mediated gene tagging in tracking IL12RB1 localization? 09/18/2021

Gene tagging allows real-time tracking of IL12RB1 subcellular localization.

Can you elaborate on the use of RNA-seq analysis to profile gene expression changes upon IL12RB1 activation? 07/02/2021

RNA-seq profiles gene expression changes upon IL12RB1 activation.

What are the applications of ChIP-seq in exploring IL12RB1's role in gene regulation? 01/23/2021

ChIP-seq investigates IL12RB1's impact on gene regulation networks.

What methods can be employed to assess IL12RB1 dimerization and its effect on downstream signaling? 06/13/2020

Crosslinking and immunoprecipitation assess IL12RB1 dimerization and signaling.

What techniques are suitable for studying IL12RB1's interaction with IL12A and IL23A? 02/14/2020

Co-immunoprecipitation and ELISA assay IL12RB1 interactions with cytokines.

How can the role of IL12RB1 in signal transduction be elucidated using knockdown experiments? 12/27/2019

Knockdown experiments reveal IL12RB1's contribution to signal transduction.

What are the advantages of using siRNA screening to identify IL12RB1-regulated pathways? 02/22/2019

siRNA screening identifies pathways influenced by IL12RB1 expression.

How do co-crystallization studies aid in understanding the molecular details of IL12RB1 interactions? 01/02/2019

Co-crystallization offers structural insights into IL12RB1 interactions.

How does flow cytometry provide insights into IL12RB1 expression levels on different cell types? 10/26/2018

Flow cytometry quantifies IL12RB1 levels on diverse cell populations.

What experimental approaches validate IL12RB1's involvement in cytokine-mediated immune responses? 06/25/2018

Functional assays validate IL12RB1's role in immune responses.

Customer Reviews (3)

Write a review
Reviews
04/06/2022

    Reliable in co-immunoprecipitation.

    10/18/2021

      Effective in asthma models.

      12/21/2017

        great in Western blot analysis.

        Ask a Question for All IL12RB1 Products

        Required fields are marked with *

        My Review for All IL12RB1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends