Active Recombinant Human IL17C protein, His-tagged

• Cat.No.: IL17C-1474H
• Product Overview: Active Recombinant Human IL17C protein(Q9P0M4)(MG+19-197 aa+LEHHHHHH), fused with C-His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: MG+19-197 aa+LEHHHHHH
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown and this effect was in a dose dependent manner.
• Molecular Mass: 21.kDa
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: IL17C interleukin 17C [ Homo sapiens ]
• Official Symbol: IL17C
• Synonyms: IL17C; interleukin 17C; interleukin-17C; CX2; IL 17C; IL 21; MGC126884; MGC138401; cytokine CX2; IL-21; IL-17C
• Gene ID: 27189
• mRNA Refseq: NM_013278
• Protein Refseq: NP_037410
• MIM: 604628
• UniProt ID: Q9P0M4