Active Recombinant Human IL1B protein

• Cat.No.: IL1B-027E
• Product Overview: Active Recombinant Human IL1B protein(NP_000567.1) (Ala 117-Ser 269) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 117-269 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/mL (100μl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL. 3. Measured by its binding ability in a functional ELISA. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 μg/mL.
• Molecular Mass: The recombinant human IL1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL1B interleukin 1, beta [ Homo sapiens ]
• Official Symbol: IL1B
• Synonyms: IL1B; interleukin 1, beta; interleukin-1 beta; IL 1B; IL1 BETA; IL1F2; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; IL-1; IL1-BETA
• Gene ID: 3553
• mRNA Refseq: NM_000576
• Protein Refseq: NP_000567
• MIM: 147720
• UniProt ID: P01584