Active Recombinant Human IL1R1 protein, hFc-tagged
|Product Overview :||Recombinant Human IL1R1 protein(NP_000868.1)(Met1-Thr332), fused with hFc tag, was expressed in HEK293.|
- Gene Information
- Related Products
|Form :||Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.|
|Bio-activity :||Measured by its ability to bind human IL1B in a functional ELISA.|
|Molecular Mass :||The recombinant human IL1R1/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 553 amino acids after removal of the signal peptide and has a predicted molecular mass of 63 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80-90 kDa due to glycosylation.|
|Protein Length :||Met1-Thr332|
|Endotoxin :||< 1.0 EU per μg of the protein as determined by the LAL method|
|Purity :||> 90 % as determined by SDS-PAGE|
|Storage :||Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.|
|Gene Name :||IL1R1 interleukin 1 receptor, type I [ Homo sapiens ]|
|Official Symbol :||IL1R1|
|Synonyms :||IL1R1; interleukin 1 receptor, type I; IL1R, IL1RA; interleukin-1 receptor type 1; CD121A; D2S1473; IL-1R-1; IL-1RT1; IL-1RT-1; antigen CD121a; interleukin receptor 1; interleukin-1 receptor alpha; interleukin-1 receptor type I; CD121 antigen-like family member A; interleukin 1 receptor alpha, type I; P80; IL1R; IL1RA; IL-1R-alpha;|
|Gene ID :||3554|
|mRNA Refseq :||NM_000877|
|Protein Refseq :||NP_000868|
|UniProt ID :||P14778|
|◆ Recombinant Protein|
|IL1R1-02H||Active Recombinant Human IL1R1 Protein (19-328aa), C-His-tagged||+Inquiry|
|IL1R1-3147H||Recombinant Human IL1R1 Protein, His (Fc)-Avi-tagged||+Inquiry|
|IL1R1-164H||Recombinant Human IL1R1 Protein (ECD), His-tagged(C-ter)||+Inquiry|
|Il1r1-10581M||Recombinant Mouse Il1r1 Protein, His (Fc)-Avi-tagged||+Inquiry|
|IL1R1-064H||Recombinant Human IL1R1 Protein, C-His-tagged||+Inquiry|
|IL1R1-1120HCL||Recombinant Human IL1R1 cell lysate||+Inquiry|
|IL1R1-1787MCL||Recombinant Mouse IL1R1 cell lysate||+Inquiry|
|IL1R1-1935RCL||Recombinant Rat IL1R1 cell lysate||+Inquiry|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Live-cell imaging techniques track IL1R1's movement on the cell surface, revealing how cellular localization impacts its responsiveness to ligands.
Advanced computational modeling and molecular dynamics simulations predict allosteric changes in IL1R1's intracellular domain upon ligand binding, revealing insights into its downstream signaling cascade.
FRET and cross-linking assays reveal the multimeric assembly of IL1R1, shedding light on its dimerization and oligomerization dynamics.
Confocal microscopy and flow cytometry visualize IL1R1's endocytosis and recycling, offering insights into its intracellular trafficking dynamics and signaling duration.
Co-immunoprecipitation assays unravel the interactions between IL1R1 and adapter proteins, elucidating the molecular details of its intracellular signaling cascade.
Mutagenesis studies dissect the interfaces between IL1R1 and accessory proteins like IL1RAP, uncovering their roles in ligand binding and signaling modulation.
Cryo-electron microscopy captures IL1R1's structural shifts during ligand binding, unveiling conformational changes that influence its ligand recognition.
Proteomics-based studies uncover the crosstalk between IL1R1 and other receptors in signaling networks, unveiling their interconnected pathways.
CRISPR-based approaches manipulate IL1R1 expression and alternative splicing, providing functional insights into the complex regulatory mechanisms governing its variations.
Phosphoproteomics characterizes IL1R1's phosphorylation landscape, revealing how post-translational modifications shape its downstream signaling outcomes.
Customer Reviews (3)Write a review
High stability. -
Suitable for ELISA. -
Effective in signaling studies. -
Ask a Question for All IL1R1 Products
Required fields are marked with *
My Review for All IL1R1 Products
Required fields are marked with *