Active Recombinant Human IL1RAP protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL1RAP-0264H
• Product Overview: Biotinylated Recombinant Human IL1RAP protein(Ser21-Glu359), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human Anti-IL1RAP Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-1 RAcP / IL-1 R3,Fc,Avitag with a linear range of 0.5-16 ng/mL.Immobilized Biotinylated Human IL-1 RAcP / IL-1 R3,Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Anti-IL1RAP Human IgG1 with a linear range of 0.2-4 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag)The protein has a calculated MW of 66 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser21-Glu359
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: Ser21-Glu359

Gene Information

• Gene Name: IL1RAP interleukin 1 receptor accessory protein [ Homo sapiens ]
• Official Symbol: IL1RAP
• Synonyms: IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein; C3orf13; IL 1RAcP; IL1R3; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta; IL-1RAcP; FLJ37788
• Gene ID: 3556
• mRNA Refseq: NM_001167928
• Protein Refseq: NP_001161400
• MIM: 602626
• UniProt ID: Q9NPH3

Reviews

05/25/2021

High solubility.

07/22/2019

Good for intracellular staining.

05/13/2018

Effective in apoptosis assays.

Q&As

How can single-cell RNA sequencing and proteomics uncover IL1RAP's interactions with immune receptors within complex networks?

Single-cell RNA sequencing and proteomics unveil IL1RAP's interactions in networks.

Can genome-wide mapping and functional assays reveal the epigenetic regulation and context-specific expression of the IL1RAP gene?

Genome mapping and assays unveil IL1RAP's epigenetic control.

How can we unravel IL1RAP's varied distribution across cell types and tissues, clarifying its functional versatility?

Innovative methods clarify IL1RAP's distribution diversity, revealing its functional adaptability.

How do advanced imaging and genetic knockout models shed light on IL1RAP's role in immune cell dynamics and functions?

Advanced imaging and genetic models illuminate IL1RAP's immune role.

What insights do computational simulations and in vitro experiments provide into IL1RAP's interaction kinetics and stability?

Simulations and experiments reveal IL1RAP's interaction details.

Do engineered cellular models clarify IL1RAP's mechanisms in inflammasome activation and cytokine release?

Engineered cell models shed light on IL1RAP's role in inflammation.

Can structural biology techniques elucidate IL1RAP's conformational changes and ligand interactions in inflammation?

Structural techniques provide insights into IL1RAP's ligand interactions.

What strategies unveil the influence of post-translational modifications on IL1RAP's localization and signaling pathways?

Strategies uncover post-translational effects on IL1RAP's signaling.

How do organ-on-chip technologies mimic tissue microenvironments to explore IL1RAP's impact on signaling pathways?

Organ-on-chip tech explores IL1RAP's impact on pathways.

What innovative methods decode IL1RAP isoforms in different cells, revealing their impact on cellular responses?

Novel techniques decode IL1RAP isoforms, exposing their influence on cell responses.