Active Recombinant Human IL2 Protein (aa 21-153), N-Fc-tagged

• Cat.No.: IL2-01H
• Product Overview: The extracellular domain of human IL-2 (aa 21-153) is fused to the N-terminus of the Fc region of human IgG4 was expressed in CHO.

Specification

• Description: This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
• Source: CHO
• Species: Human
• Tag: N-hFc
• Bio-activity: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.
• Molecular Mass: ~40 kDa (SDS-PAGE)
• Protein Length: 21-153
• Endotoxin: <5 EU/mg protein (LAL test; Lonza).
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 1 year after receipt when stored at -20 centigrade.
  Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Storage: Short Term Storage: +4 centigrade
  Long Term Storage: -20 centigrade
• Storage Buffer: Lyophilized from 0.2 μm-filtered solution in PBS.
• Reconstitution: Reconstitute 50 μg vial in 50 μL sterile water.
  Add 1 × PBS to the desired protein concentration.
• Handling: Avoid freeze/thaw cycles.
  Centrifuge lyophilized vial before opening and reconstitution.
• Shipping: BLUE ICE

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens (human) ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; IL-2; TCGF; lymphokine; interleukin-2; T cell growth factor; aldesleukin; involved in regulation of T-cell clonal expansion
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568