| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Avi&His |
| Protein Length : |
AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) |
| Description : |
Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. |
| Predicted N Terminal : |
His (IL23A) & Ile 23 (IL12B) |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Bio-activity : |
Immobilized Anti-p40 subunit of IL23（Anti-IL12B） MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL23A & IL12B, His Tag with a linear range of 4.9-39 ng/mL. Immobilized Anti-p19 subunit of IL23 （Anti-IL23A） MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL23A & IL12B, His Tag with a linear range of 1.2-19.5 ng/mL. |
| Molecular Mass : |
Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The protein has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). The protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
