| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Fc-Avi |
| Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Biotinylated Human IL-23 R, Fc,Avitag at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with a linear range of 39-156 ng/mL.Immobilized Biotinylated Human IL-23 R, Fc,Avitag at 5 μg/mL (100 μL/well), can bind pre-mixed increasing concentrations of Human IL23A/p19 Neutralizing Antibody and 0.25 μg/mL (100 μL/well) Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with a half maximal inhibitory concentration (IC50) of 0.3889 μg/mL. |
| Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Proteinlength : |
Gly24-Gly355 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
