Active Recombinant Human IL33 protein, FLAG-tagged

• Cat.No.: IL33-29791TH
• Product Overview: Recombinant Human IL33(aa 112-270) fused with FLAG tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 112-270 a.a.
• Description: Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
• Form: Liquid. 0.2μm-filtered solution in PBS, pH 7.2.
• Bio-activity: Activates human ST2-dependent NF-κB pathway.
• Molecular Mass: ~23kDa (SDS-PAGE)
• Endotoxin: <0.1EU/μg purified protein (LAL test; Lonza).
• Purity: ≥90% (SDS-PAGE)
• Stability: Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Storage: Short Term Storage +4 centigrade;Long Term Storage -20 centigrade;After opening, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles.
• Concentration: 1mg/ml

Gene Information

• Gene Name: IL33 interleukin 33 [ Homo sapiens ]
• Official Symbol: IL33
• Synonyms: IL33; interleukin 33; C9orf26, chromosome 9 open reading frame 26 (NF HEV); interleukin-33; DKFZp586H0523; DVS27; DVS27 related protein; IL1F11; interleukin 1 family; member 11; NF HEV; nuclear factor for high endothelial venules; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor from high endothelial venules; NF-HEV; NFEHEV; C9orf26; RP11-575C20.2
• Gene ID: 90865
• mRNA Refseq: NM_001199640
• Protein Refseq: NP_001186569
• MIM: 608678
• UniProt ID: O95760
• Chromosome Location: 9p24.1
• Pathway: Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Influenza A, organism-specific biosystem; Influenza A, conserved biosystem
• Function: cytokine activity; protein binding