Active Recombinant Human IL7R Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: IL7R-130H
• Product Overview: Biotinylated Recombinant Human IL7R Protein (Glu 21 - Gly 236) (P16871), fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Glu 21 - Gly 236
• Predicted N Terminal: Glu 21
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL-7, Tag Free at 10μg/mL (100 μL/well) can bind Biotinylated Human IL-7 R alpha, Fc Tag with a linear range of 0.02-0.15 μg/mL.
• Molecular Mass: Recombinant Human IL7R Protein has a calculated MW of 53.3 kDa. As a result of glycosylation, The protein migrates as 70-100 kDa under reducing (R) condition and 130-200 kDa under non-reducing (NR) condition on SDS-PAGE gel.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: IL7R interleukin 7 receptor [ Homo sapiens ]
• Official Symbol: IL7R
• Synonyms: IL7R; interleukin 7 receptor; interleukin-7 receptor subunit alpha; CD127; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; ILRA; IL7RA; CDW127; IL-7R-alpha
• Gene ID: 3575
• mRNA Refseq: NM_002185
• Protein Refseq: NP_002176
• MIM: 146661
• UniProt ID: P16871
• Chromosome Location: 5p13
• Pathway: Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-7 Signaling Pathway, organism-specific biosystem; Immune System, organism-specific biosystem
• Function: antigen binding; interleukin-7 receptor activity; protein binding; receptor activity