Active Recombinant Human Insulin-like Growth Factor Binding Protein 3

• Cat.No.: IGFBP3-145H
• Product Overview: Recombinant Human Insulin-like Growth Factor Binding Protein 3 DNA sequence encoding the human Insulin-like Growth Factor Binding Protein 3 sequence (containing the signal peptide sequence, and the mature human IGFBP-3 sequence) was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Insulin-like Growth Factor Binding Protein 3 is a member of the insulin-like growth factor binding protein (IGFBP) family. This family includes IGFBP-1 to IGFBP-6 that bind to the insulin-like growth factors (IGFs), thus regulating the half-life, activity, transport, and tissue distribution of the IGFs. Structurally, the IGFBP family of proteins is defined by highly homologous amino and carboxy terminal domains with a structurally diverse central region.
• Amino Acid Sequence: GASSGGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK.
• Molecular Mass: Insulin-like Growth Factor Binding Protein 3 migrates as a broad band between 40 and 45 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IGFBP-3 that has a predicted molecular mass of 28.7 kDa.
• pI: Insulin-like Growth Factor Binding Protein 3 separates into a number of isoforms with a pI between 6.8 and 8.2 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IGFBP-3 that has a predicted pI of 8.76.
• % Carbohydrate: Purified Insulin-like Growth Factor Binding Protein 3 consists of 25-35% carbohydrate by weight.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: The ED50 of Insulin-like Growth Factor Binding Protein 3 is typically 0.13-0.20 μg/ml as measured by its ability to neutralize rhIGF-II mediated proliferation of the MCF-7 adenocarcinoma cell line.

Gene Information

• Gene Name: IGFBP3 insulin-like growth factor binding protein 3 [ Homo sapiens ]
• Synonyms: IGFBP3; insulin-like growth factor binding protein 3; IBP3; BP-53; binding protein 29; IGF-binding protein 3; growth hormone- dependent binding protein; acid stable subunit of the 140 K IGF complex
• Gene ID: 3486
• mRNA Refseq: NM_000598
• Protein Refseq: NP_000589
• UniProt ID: P17936
• Chromosome Location: 7p13-p12
• MIM: 146732
• Pathway: p53 signaling pathway; Diabetes pathways
• Function: insulin-like growth factor I binding; metal ion binding; protein tyrosine phosphatase activator activity