| Cat. No. : |
IGFBP3-145H |
| Description : |
Insulin-like Growth Factor Binding Protein 3 is a member of the insulin-like growth factor binding protein (IGFBP) family. This family includes IGFBP-1 to IGFBP-6 that bind to the insulin-like growth factors (IGFs), thus regulating the half-life, activity, transport, and tissue distribution of the IGFs. Structurally, the IGFBP family of proteins is defined by highly homologous amino and carboxy terminal domains with a structurally diverse central region. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
GASSGGLGPVVRCEPCDARALAQCA PPPAVCAELVREPGCGCCLTCALSE GQPCGIYTERCGSGLRCQPSPDEAR PLQALLDGRGLCVNASAVSRLRAYL LPAPPAPGNASESEEDRSAGSVESP SVSSTHRVSDPKFHPLHSKIIIIKK GHAKDSQRYKVDYESQSTDTQNFSS ESKRETEYGPCRREMEDTLNHLKFL NVLSPRGVHIPNCDKKGFYKKKQCR PSKGRKRGFCWCVDKYGQPLPGYTT KGKEDVHCYSMQSK. |
| Molecular Mass : |
Insulin-like Growth Factor Binding Protein 3 migrates as a broad band between 40 and 45 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IGFBP-3 that has a predicted molecular mass of 28.7 kDa. |
| pI : |
Insulin-like Growth Factor Binding Protein 3 separates into a number of isoforms with a pI between 6.8 and 8.2 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IGFBP-3 that has a predicted pI of 8.76. |
| % Carbohydrate : |
Purified Insulin-like Growth Factor Binding Protein 3 consists of 25-35% carbohydrate by weight. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50 of Insulin-like Growth Factor Binding Protein 3 is typically 0.13-0.20 μg/ml as measured by its ability to neutralize rhIGF-II mediated proliferation of the MCF-7 adenocarcinoma cell line. |
