Active Recombinant Human ITGA2&ITGB1 protein, His-tagged

• Cat.No.: ITGA2&ITGB1-1594H
• Product Overview: Recombinant Human ITGA2&ITGB1 protein(Tyr30-Thr1132(ITGA2)&Gln21-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.078-1.25 μg/mL.
• Molecular Mass: Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.7 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 150-160 kDa (ITGA2) and 105-135 kDa (ITGB1) respectively due to glycosylation.
• Proteinlength: Tyr30-Thr1132(ITGA2)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA2 integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) [ Homo sapiens ]
• Official Symbol: ITGA2
• Synonyms: ITGA2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); CD49B; integrin alpha-2; CD49b; integrin alpha 2; collagen receptor; VLA-2 subunit alpha; platelet antigen Br; platelet glycoprotein Ia; platelet glycoprotein GPIa; CD49 antigen-like family member B; platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; BR; GPIa; VLA-2; VLAA2; BDPLT9
• Gene ID: 3673
• mRNA Refseq: NM_002203
• Protein Refseq: NP_002194
• MIM: 192974
• UniProt ID: P17301