Active Recombinant Human ITGA3&ITGB1 protein, His-tagged

• Cat.No.: ITGA3&ITGB1-1566H
• Product Overview: Recombinant Human ITGA3&ITGB1 protein(Phe33-Glu991(ITGA3)&Gln21-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 33-991;21-728 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.1-8 ng/mL.
• Molecular Mass: Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA3 and ITGB1, has a calculated MW of 133.3 kDa (ITGA3) and 83.7 kDa (ITGB1). Subunit ITGA3 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 125-150 kDa (ITGA3) and 100-110 kDa (ITGB1) respectively due to glycosylation.
• Proteinlength: Phe33-Glu991(ITGA3)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA3 integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) [ Homo sapiens ]
• Official Symbol: ITGA3
• Synonyms: ITGA3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); antigen identified by monoclonal J143 , MSK18; integrin alpha-3; CD49c; GAP B3; VCA 2; VLA3a; FRP-2; galactoprotein B3; VLA-3 subunit alpha; CD49 antigen-like family member C; antigen identified by monoclonal J143; very late activation protein 3 receptor, alpha-3 subunit; VL3A; CD49C; GAPB3; MSK18; VCA-2; GAP-B3; FLJ34631; FLJ34704
• Gene ID: 3675
• mRNA Refseq: NM_002204
• Protein Refseq: NP_002195
• MIM: 605025
• UniProt ID: P26006