Active Recombinant Human ITGA4 & ITGB1 Heterodimer Protein, His-tagged

• Cat.No.: ITGA4&ITGB1-356H
• Product Overview: Recombinant Human ITGA4 & ITGB1 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)). Predicted N-terminus: Tyr 34 (ITGA4) & Gln 21 (ITGB1). Human ITGA4 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Tyr 34 (ITGA4) & Gln 21 (ITGB1). The reducing (R) protein migrates as 60-70 kDa and 80-85 kDa (ITGA4) and 100-133 kDa and 135-150 kDa (ITGB1) respectively due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Recombinant Human ITGA4 & ITGB1 Heterodimer Protein, His-tagged with a linear range of 1-16 ng/mL (QC tested).
• Molecular Mass: 111.4 kDa (ITGA4) & 83.7 kDa (ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) [ Homo sapiens ] & ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: ITGA4 & ITGB1
• Synonyms: ITGA4; CD49D; integrin alpha-4; CD49d; 269C wild type; IA4; MGC90518; & ITGB1; FNRB, MDF2, MSK12; integrin beta-1; CD29; GPIIA; FNRB; MDF2; VLAB; MSK12; VLA-BETA
• Gene ID: 3676 & 3688
• mRNA Refseq: NM_000885 & NM_002211
• Protein Refseq: NP_000876 & NP_002202
• MIM: 192975 & 135630
• UniProt ID: P13612 & P05556