| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
39-1012;21-728 a.a. |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. |
| Bio-activity : |
Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.016-0.5 μg/mL. |
| Molecular Mass : |
Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA8 and ITGB1, has a calculated MW of 114.6 kDa (ITGA8) and 83.7 kDa (ITGB1). Subunit ITGA8 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-170 kDa (ITGA8) and 100-115 kDa (ITGB1) respectively due to glycosylation. |
| Proteinlength : |
Phe39-Leu1012(ITGA8)&Gln21-Asp728 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
