Active Recombinant Human ITGAE&ITGB7 protein, His-tagged

• Cat.No.: ITGAE&ITGB7-0192H
• Product Overview: Recombinant Human ITGAE&ITGB7 protein(Phe19-Ser1124(ITGAE)&Glu20-His723(ITGB7)), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-1124;20-723 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free immobilized on CM5 Chip can bind Human E-Cadherin, Fc Tag with an affinity constant of 0.148 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: The reducing (R) protein migrates as 135-150 kDa (ITGAE) and 100-115 kDa (ITGB7) respectively due to glycosylation.
• Proteinlength: Phe19-Ser1124(ITGAE)&Glu20-His723(ITGB7)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGAE integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) [ Homo sapiens ]
• Official Symbol: ITGAE
• Synonyms: ITGAE; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin alpha-E; CD103; HUMINAE; HML-1 antigen; integrin alpha-IEL; mucosal lymphocyte 1 antigen; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; MGC141996
• Gene ID: 3682
• mRNA Refseq: NM_002208
• Protein Refseq: NP_002199
• MIM: 604682
• UniProt ID: P38570