Active Recombinant Human ITGAV&ITGB1 protein, His-tagged
Cat.No. : | ITGAV&ITGB1-1342H |
Product Overview : | Recombinant Human ITGAV&ITGB1 protein(Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1)), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant. |
Bio-activity : | Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 4-78 ng/mL. |
Molecular Mass : | Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.9 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 125-150 kDa (ITGAV) and 95-110 kDa (ITGB1) respectively due to glycosylation. |
Protein Length : | Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | ITGAV integrin, alpha V [ Homo sapiens ] |
Official Symbol : | ITGAV |
Synonyms : | ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142; |
Gene ID : | 3685 |
mRNA Refseq : | NM_001144999 |
Protein Refseq : | NP_001138471 |
MIM : | 193210 |
UniProt ID : | P06756 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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