Active Recombinant Human ITGAV & ITGB5 Heterodimer protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: ITGAV & ITGB5-758H
• Product Overview: Biotinylated Recombinant Human ITGAV & ITGB5 Heterodimer protein(P06756-1(ITGAV) & P18084(ITGB5))(31-992 aa(ITGAV) & 24-719 aa(ITGB5)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 31-992 aa(ITGAV) & 24-719 aa(ITGB5)
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS, 8% trehalose (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human ITGAV&ITGB5, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 114 kDa (ITGAV) & 81.4 kDa (ITGB5). Due to glycosylation, the protein migrates to 85-140 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142
• Gene ID: 3685
• mRNA Refseq: NM_001144999
• Protein Refseq: NP_001138471
• MIM: 193210
• UniProt ID: P06756
• Gene Name 2: ITGB5 integrin, beta 5 [ Homo sapiens ]
• Official Symbol 2: ITGB5
• Synonyms 2: ITGB5; integrin, beta 5; integrin beta-5; FLJ26658
• Gene ID 2: 3693
• mRNA Refseq 2: NM_002213
• Protein Refseq 2: NP_002204
• MIM 2: 147561
• UniProt ID 2: P18084