Active Recombinant Human JAK2 Protein, Flag tagged

• Cat.No.: JAK2-04H
• Product Overview: Recombinant JAK2 (532-1132) protein that includes amino acids 532-1132 of human JAK2 protein (accession number NP_004963.1) was expressed in a baculovirus expression system and contains an N-terminal FLAG-Tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag
• Protein Length: 532-1132 aa
• Description: JAK2 (Janus Kinase 2), also known as Tyrosine-Protein Kinase JAK2 or JTK10, is a non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. In the cytoplasm, JAK2 plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO);or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, JAK2 can phosphorylate specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Then it can subsequently phosphorylate the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. JAK2 signaling is implicated in diverse biological processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and alteration of heterochromatin.
• Form: Recombinant JAK2 (532-1132) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.
• Molecular Mass: 71 kDa
• Purity: >90%
• Applications: Recombinant JAK2 (532-1132) protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.2 μg/μL

Gene Information

• Gene Name: JAK2 Janus kinase 2 [ Homo sapiens (human) ]
• Official Symbol: JAK2
• Synonyms: JAK2; Janus kinase 2; tyrosine-protein kinase JAK2; JTK10; JAK-2; Janus kinase 2 (a protein tyrosine kinase); THCYT3
• Gene ID: 3717
• mRNA Refseq: NM_004972
• Protein Refseq: NP_004963
• MIM: 147796
• UniProt ID: O60674