Species : |
Human |
Source : |
Insect Cells |
Tag : |
Fc |
Description : |
Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occuring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin. |
Form : |
Lyophilized |
Bio-activity : |
The activity of sVEGFR-2/Fc was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 10-30 ng/ml. |
Purity : |
> 90 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Characteristic : |
Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. |
Storage : |
Lyophilized VEGFR-2 Fc/Chimera protein although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution FLK1 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Storage Buffer : |
KDR fusion protein was lyophilized from a concentrated (1 mg/mL) sterile solution containing 1 × PBS pH 7.4. |
Reconstitution : |
It is recommended to reconstitute the lyophilized VEGFR2 in sterile water not less than 100 μg/mL, which can then be further diluted to other aqueous solutions. |