Active Recombinant Human Killer Cell Immunoglobulin-Like Receptor, Three Domains, Long Cytoplasmic Tail, 2, Fc-tagged

• Cat.No.: KIR3DL2-5669H
• Product Overview: RecombinantHuman KIR3DL2 protein was expressed in Murine myeloma cell line with the Fcpart of Human IgG1 Pro100-Lys330 (C-terminus) and Human KIR3DL2 Leu22-Leu339(N-terminus).

Specification

• Cat. No.: KIR3DL2-5669H
• Description: KIR3DL2is a type I transmembrane protein of the p70 family of killer cell Ig-likereceptors (KIR). KIR are expressed on CD56 dim NK cells and T cell subsetswhere they participate in identifying normal and abnormal cells andregulating effector functions of the innate immune system. KIR are named forthe number of Ig-like domains (2D or 3D) in the extracellular domain (ECD)and whether they have long or short (L, S) cytoplasmic tail. As with other inhibitoryKIR, KIR3DL2 has two ITIM domains within its long tail (3). KIR3DL2 isdiverse, with twelve alleles identified and as many as five single amino acid(aa) polymorphisms found in a single individual.
• Source: Mammalian cells
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminalSequence: Leu22
• MolecularWeight: 61.4 kDa (monomer)
• Activity: Measuredby its ability to bind MDA-MB-231 human breast adenocarcinoma cells in a flowcytometry assay. A 50-100 fold increase in fluorescence intensity can be observedwhen MDA-MB-231 cells are stained with 1 μg/mL rhKIR3DL2/Fc Chimera and goatanti-human Fc-PE as compared to cells stained with goat anti-human Fc-PE.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 85-90kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: KIR3DL2 killer cellimmunoglobulin-like receptor, three domains, long cytoplasmic tail, 2 [ Homosapiens ]
• Official Symbol: KIR3DL2
• Synonyms: KIR3DL2; killer cellimmunoglobulin-like receptor, three domains, long cytoplasmic tail, 2; p140;NKAT4; CD158K; NKAT-4; NKAT4B; killer cell immunoglobulin-like receptor 3DL2;KIR antigen 3DL2; killer Ig receptor; p70 NK receptor CL-5; MHC class I NKcell receptor; CD158 antigen-like family member K; p70 killer cell inhibitoryreceptor; natural killer-associated transcript 4; natural killer cellinhibitory receptor; p70 natural killer cell receptor clone CL-5; killer cellimmunoglobulin-like receptor KIR3DL2
• Gene ID: 3812
• mRNA Refseq: NM_001242867
• Protein Refseq: NP_001229796
• MIM: 604947
• UniProt ID: P43630
• Chromosome Location: 19q13.4
• Pathway: Adaptive ImmuneSystem; Antigen processing and presentation; Graft-versus-host disease; ImmuneSystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoidcell; Natural killer cell mediated cytotoxicity
• Function: receptor activity