Active Recombinant Human Killer Cell Lectin-Like Receptor Subfamily F, Member 1, Fc-tagged

• Cat.No.: KLRF1-5672H
• Product Overview: Recombinant Human KLRF1protein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (N-terminus) and Val66-Tyr231 (C-terminus).

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 66-231 a.a.
• Description: KLRF1 is an 80 kDatype II transmembrane, homodimeric, activating coreceptor expressedexclusively on the surface of NK cells. Human KLRF1, a member of the C-typelectin family of proteins, is 231 aa in length with a 38 amino acid (aa)cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellulardomain (ECD). The ECD contains the C-type lectin like domain (aa 114-230) andfour potential sites of N-linked glycosylation. Multiple splicing variantsproduce four isoforms for KLRF1.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Met
• Molecular Weight: 45.8 kDa
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of U937human histiocytic lymphoma cells. Welte, S. et al. (2006) Nat. Immunol. 7:12.rhKLRF1/Fc Chimera, immobilized at 5 μg/mL (100 μL/well), can induce greaterthan 40% of U937 cell adhesion.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 59-62kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: KLRF1 killer cell lectin-likereceptor subfamily F, member 1 [ Homo sapiens ]
• Official Symbol: KLRF1
• Synonyms: KLRF1; killer celllectin-like receptor subfamily F, member 1; NKp80; CLEC5C; MGC119907;MGC119908; MGC119909; lectin-like receptor F1; activating coreceptor NKp80;killer cell lectin-like receptor F1; C-type lectin domain family 5 member C;killer cell lectin-like receptor subfamily F member 1; ML;OTTHUMP00000238675; OTTHUMP00000238676; OTTHUMP00000238678
• Gene ID: 51348
• mRNA Refseq: NM_016523
• Protein Refseq: NP_057607
• MIM: 605029
• UniProt ID: Q9NZS2
• Chromosome Location: 12p13.31
• Function: MHC class I receptoractivity; binding; receptor activity; sugar binding; transmembrane signalingreceptor activity