Active Recombinant Human KLK1 protein, His-tagged

• Cat.No.: KLK1-299H
• Product Overview: Active Recombinant Human KLK1 protein(P06870-1)(Ile25-Ser262) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-262 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl, 8% trehalose (pH 7.5).
• Bio-activity: Immobilized Human Kallikrein 1 (active form), His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Kallikrein 1 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLK1 kallikrein 1 [ Homo sapiens ]
• Official Symbol: KLK1
• Synonyms: KLK1; kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein-1; Klk6; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kidney/pancreas/salivary gland kallikrein; hK1; KLKR
• Gene ID: 3816
• mRNA Refseq: NM_002257
• Protein Refseq: NP_002248
• UniProt ID: P06870